breast cancer cell lines t47d  (ATCC)

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: Synergistic cytotoxic effect of 17-AAG-loaded PEG-CS nanoparticles and trastuzumab on T47D cells. Cell viability was assessed by MTT assay after 72 h of treatment. Data are presented as mean ± SD ( n = 3). The combination group (17-AAG-loaded NPs + trastuzumab) showed significantly reduced cell viability (15%) compared to treatment with 17-AAG-loaded NPs alone (50%), trastuzumab alone (35%), or blank NPs + trastuzumab (100%). Statistical significance: **** p < 0.0001 vs. Blank NPs + Trastuzumab group (one-way ANOVA with Tukey’s post-hoc test). The Combination Index (CI) calculated using CompuSyn software was 0.72, indicating strong synergy.

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: MTT Assay, Software

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: In vitro cytotoxicity of 17-AAG-loaded PEG-CS NPs in T47D and MDA-MB-231 cells. Cell viability after 72 h treatment with free 17-AAG or 17-AAG-loaded NPs. The nanoformulation showed significantly enhanced cytotoxicity, with an IC₅₀ of 2.3 ± 0.3 µM in T47D cells, representing a threefold increase compared to free 17-AAG (IC₅₀ = 6.9 ± 0.5 µM). Similar results were observed in MDA-MB-231 cells (IC₅₀ = 3.0 µM for NPs vs. 8.1 µM for free drug). Blank PEG-CS NPs showed no significant toxicity in NHDF cells (up to 50 µg/mL). Data are mean ± SEM ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test. **** P < 0.0001 vs. control.

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: In Vitro, Control

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: Flow cytometric analysis of apoptosis in T47D cells after 24 h treatment with 17-AAG-loaded PEG-CS NPs. A Untreated control cells. B Cells treated with 17-AAG-loaded PEG-CS NPs. The percentage of apoptotic cells (Q2 + Q3) increased from 16.5% in the control group to 52.2% in the treated group. Data are representative of three independent experiments ( n = 3).

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: Control

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: Cell cycle distribution in T47D cells after 24 h treatment with 17-AAG-loaded PEG-CS NPs. The treated group showed an increase in the G2/M phase (26.50%) compared to control (19.30%), indicating G2/M arrest. Data are representative of three independent experiments ( n = 3).

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: Control

Journal: Scientific Reports

Article Title: Development of a pH-responsive pegylated chitosan nanocarrier for targeted delivery of 17-AAG and synergistic therapy in HER2 + breast cancer

doi: 10.1038/s41598-025-30507-2

Figure Lengend Snippet: qRT-PCR analysis of HSP90 and β-actin gene expression in T47D cells after 72 h treatment with 17-AAG-loaded PEG-CS NPs. A Melting curves for HSP90 (Tm = 78.2 °C) and β-actin (Tm = 83.9 °C), showing single sharp peaks that confirm the specificity of amplification and absence of primer-dimers. Data are representative of three independent experiments ( n = 3). B Amplification curves for HSP90 (purple line) and β-actin (blue line) genes, showing the cycle threshold (Ct) values used for relative quantification of gene expression.

Article Snippet: The T47D human breast cancer cell line was obtained from the Pasteur Institute of Iran.

Techniques: Quantitative RT-PCR, Gene Expression, Amplification, Quantitative Proteomics